【摘要】 目的探讨针刺治疗抑郁症的的作用机制。方法将48只成年雌雄各半的SD大鼠随机分为正常组、模型组、针刺组和药物组,每组12只。后3组采用孤养和长期不可预见的中等强度刺激应激造成抑郁大鼠模型,同时针刺组和药物组开始治疗,周期21 d。每周测定各组大鼠行为学的改变,治疗结束后用放射免疫法测定大鼠血清ACTH,T3,T4,rT3和TSH的浓度。结果与正常组相比,模型大鼠行为学评分降低,血清T3,T4,rT3、TSH均有明显减低(P<0.05,P<0.01),而ACTH显著升高(P<0.01);与模型组相比,各治疗组的行为学评分升高,针刺组血清T3,rT3,TSH水平均有显著升高(P<0.05,P<0.01),ACTH水平有显著降低(P<0.01)。结论针刺干预可改变抑郁大鼠行为学异常,具有调节抑郁大鼠下丘脑?垂体?肾上腺皮质轴和下丘脑?垂体?甲状腺轴功能的作用。
Abstract:ObjectiveTo study the therapeutic effects of acupuncture on depression and to probe the mechanism. MethodsForty?eight adult SD rats, 16 females and 16 males, were randomly divided into a normal group, a model group, an acupuncture group and west medicine group, 12 rats in each group. Separated feeding. Long?term unpredictable and middle stimulation stress were used for development of depressed rat model. At the same time, the treatment groups were treated for 21days. The changes of behavior were recorded in every week. The contents of adrenocorticotrophic hormone (ACTH), TSH, T3, T4 and rT3 in the serum were detected by radio?immunity. ResultsCompared with the normal group,the times of crossing and rearing movement in model group decreased significantly from the 7th day after modeling, the contents of TSH, T3, T4 and rT3 decreased significantly, as well as the contents of ACTH increased significantly in model group. While in comparison with model group, the times of crossing and rearing movement increased significantly in acupuncture and west medicine groups;the contents of TSH, T3 and rT3 increased and ACTH decreased significantly in acupuncture and west medicine groups. ConclusionAupuncture is effective on the depressed rats. It plays the therapeutic role through ameliorate the function of hypothalamus?pituitary?adrenal axis and hypothalamus?pituitary?thyroid axis.
抑郁症是一种以持续性心境低落为主要特征的常见情感障碍疾病,甚则致死致残[1]。其发病涉及生物、心理和社会等多方面因素,机理至今尚不完全清楚。有资料[2]表明,抑郁症患者神经内分泌轴调节功能常有异常,而长期慢性应激刺激更易导致神经内分泌调节功能障碍,并导致情绪和行为的异常。针灸介入抑郁症的治疗已有近20年的历史,取得了明显的疗效。本实验旨在观察慢性应激抑郁模型大鼠日常行为学变化以及血清ACTH,T3,T4,rT3,TSH的水平,即抑郁大鼠下丘脑?垂体?肾上腺皮质轴(HPAA)和下丘脑?垂体?甲状腺轴(HPPA)功能的改变,以及针刺干预对它们的影响。现将结果报告如下。
1 材料与仪器
1.1 动物成年SD大鼠,体质量220~290 g,雌雄各半(由广州中医药大学动物中心提供,动物合格证号:2007A017,SPF级)。
1.2 试剂与仪器ACTH,T3,T4,rT3,TSH放免试剂盒由北京科美东雅生物技术有限公司提供。上海日环仪器?TSN?695B型γ放射免疫计数测定仪,RC5C低温高速离心机(美国杜邦公司),自制敞箱:不透明材料制成的立方形,长、宽各80 cm,高40 cm, 底面由面积相等、边长16 cm的正方形25块组成。
2 方法
2.1 动物分组首先用Open?field法[3]作行为学评分,选择得分相近的48只大鼠。适应性喂养7 d后,给予分组。随机分为正常组、模型组、药物组和针刺组,共4组,每组12只。正常组每笼饲养6只,雌雄分开。
2.2 模型制备按照参考文献方法[3]进行抑郁症模型大鼠的复制。模型组、针刺组和药物组每笼饲养1只。除正常组之外其余各组接受21 d各种不同的应激,包括断水24 h;断食24 h;夹尾1 min;4℃冰水游泳5 min;摇晃:1次/s,5 min;昼夜颠倒;点击足底,电压50 mV,每5 s刺激1次,间歇5 s,共刺激10次。每天随机选择一种刺激,每种刺激平均使用3次,共21 d。